Synopsis
Learn how to interpret HPLC results and why purity percentages matter for your research outcomes.
The Importance of Peptide Purity
When working with research peptides, purity is one of the most critical quality metrics. But what do those percentages really mean, and why should you care?
HPLC Analysis Explained
High-Performance Liquid Chromatography (HPLC) is the gold standard for measuring peptide purity. This analytical technique separates the target peptide from impurities and provides a quantitative measurement of purity.
What Affects Purity?
- Synthesis method: Solid-phase peptide synthesis (SPPS) can introduce various by-products
- Purification steps: Additional purification increases purity but also cost
- Storage conditions: Improper storage can degrade peptides over time
Purity Standards at BioEdge
At BioEdge Labs, we maintain a minimum purity threshold of 98% for all research peptides. Each batch is accompanied by:
- HPLC chromatogram
- Mass spectrometry (MS) data
- Certificate of Analysis (COA)
- Stability testing results
Best Practices for Researchers
To maintain peptide integrity in your lab:
- Store lyophilized peptides at -20°C or below
- Reconstitute only what you need for immediate use
- Use sterile, bacteriostatic water for reconstitution
- Avoid repeated freeze-thaw cycles
Understanding purity metrics empowers you to make informed decisions about your research materials and ensures reproducible results.
